›› 2013, Vol. 40 ›› Issue (4): 10-13.

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Effect of TCA on Gsα Expression in Peritoneal Macrophage in Mouses

GUO Wen-rui1,2, MAO Wei1,2, GUAN Hong1,2, LI Pei-feng1,2   

  1. 1. College of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China;
    2. Key Laboratory of Clinical Diagnosis and Treatment Techniques for Animal Disease,Ministry of Agriculture,Hohhot 010018,China
  • Received:2012-09-13 Online:2013-04-20 Published:2013-04-19

Abstract: The assay was aimed to explore the effect of taurocholate acid (TCA) on Gsα expression in peritoneal macrophage in mouses, and provide clues for explaining the function mechanism of TCA on mouse peritoneal macrophages. The peritoneal macrophages of mouse were isolated and cultured. The effect of TCA on the expression of Gsα mRNA was examined quantitatively by Real-time fluorescent quantitative PCR. TCA(15,1.5 and 0.15 μg/mL) decreased significantly the expression of Gsα mRNA in peritoneal macrophage in mouses (P＜0.05), compared with that in control group and LPS group. The results showed that TCA could significantly promote the expression of Gsα mRNA in peritoneal macrophage in mouses.

Key words: taurocholate acid; Gsα; Real-time fluorescent quantitative PCR; peritoneal macrophage

CLC Number: