牛传染性鼻气管炎病毒gD蛋白主要抗原区域的高效表达、纯化与鉴定

Abstract

Abstract: According to the published IBRV genome sequence, we designed and synthetized one pair of specific primers, amplied about 846 bp gD gene fragment. The fragment was cloned into prokaryotic expression vector pET-30a, after enzyme digestion and DNA sequencing were correct, the recomninated vector was transformed into BL21(DE3) bacteria and induced by IPTG, recombinant protein was expressed in partially soluble form. After purification of recombinant protein, Western blotting proved that it had good antigenicity and specificity. The present study using prokaryotic expression system had good antigenicity of gD protein, which laid a material foundation for IBRV further research and development of diagnostic reagents.

Key words: infectious boviner rihinotracheitis virus; gD protein; prokaryotic expression; identification

CLC Number:

NAI De-he. Truncated Expression, Purification and Identification of gD Protein of Infectious Bovine Rihinotracheitis Virus[J]. , 2013, 40(3): 77-79.

References

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Truncated Expression, Purification and Identification of gD Protein of Infectious Bovine Rihinotracheitis Virus

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