催乳复合物、胰岛素样生长因子-Ⅰ对奶牛乳腺上皮细胞中AKT-1和mTOR表达的作用

Abstract

Abstract: Signal transduction molecules AKT-1 and mTOR were essential for milk protein gene transcription and translation in mammary epithelial cells.Insulin,dexamethasone and prolactin composed of prolactin complex (DIP), the variety of lactation signal pathway activation was a necessary condition,including AKT-1/mTOR. In this study,the qRT-RCR and Western blotting were used to test above-mentioned two genes's expression level of mRNA and protein in different time points.The results showed that IGF-Ⅰ promoted significantly the expression levels of mRNA and protein for AKT-1 gene in the basis of DIP compared to the absence of IGF-Ⅰ(P<0.05), and it also promoted significantly the expression of mTOR gene in mRNA and protein levels(P<0.05).

Key words: mammary epithelial cells; IGF-Ⅰ; DIP; mRNA; protein

CLC Number:

S816.79

SHI Lin-lin, ZHAO Feng, GAO Xue-jun, BI Wei-wei, WU Cai-xia, LI Qing-zhang. Effection of DIP and IGF-Ⅰ on the Expression of AKT-1 and mTOR Genes in Bovine Mammary Epithelial Cells[J]. , 2013, 40(3): 23-27.

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Effection of DIP and IGF-Ⅰ on the Expression of AKT-1 and mTOR Genes in Bovine Mammary Epithelial Cells

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