Abstract: Region A of fibronectin-binding protein A (FnbpA) was amplified by PCR and the recombinant expression vector pVAX-SFn was constructed.Expressed protein was analyzed and identified by Western blotting and ELISA. Then the recombinant plasmid was used to immune C57BL/6 mice and induced antibody, T lymphocyte proliferative response and experiment mice challenge test were measured. The results showed that cellular and humoral immune responses induced by recombinant plamid were much stronger than the control one. The challenge experiment in immunized C57BL/6 mice indicated that the recombinant eukaryotic plasmid based on FnbpA could induce immune response effectively in mice. This result built a good foundation for practical use of this DNA vaccine in future.

Key words: Staphylococcus aureus; fibronectin-binding protein; DNA vaccine; immune response