piggyBac转座子在哺乳动物中的应用

Abstract

Abstract: Since the resurrection of Sleeping Beauty(SB11), piggyBac(PB) is also described to work in mammals. DNA transposons are mobile genetic units identified in many mammals, can change the structures of genome and influence the function of special gene. The exogenous gene can be inserted into a new locus by transposase following a 'cut and paste' mechanism, so transposons have been widely used as tools to study the gene function, generate transgenic animals, and research gene therapy. Compared with SB11, PB has higher transposition level, no dose inhibition effect, and can carry larger fragments, thus PB has been cared more and more recently. Herein, we describe its mechanism of action,the factors influencing the transgenic effects,and the application in mammals.

Key words: piggyBac; transposon; transgene

CLC Number:

YANG Guan-heng, ZENG Fan-yi. Application of PiggyBac Transposon in Mammals[J]. , 2013, 40(2): 31-35.

References

1 Belay E, Dastidar S, Vanden Driessche T, et al. Transposon-mediated gene transfer into adult and induced pluripotent stem cells. J Curr Gene Ther,2011,11(5):406～413.
2 Berghammer A J, Klingler M, Wimmer E A. A universal marker for transgenic insects. J Nature, 1999, 402(6760):370～371.
3 Cadianos J, Bradley A. Generation of an inducible and optimized piggyBac transposon system. J Nucleic Acids Res, 2007,35(12):e87.
4 Cary L C, Goebel M, Corsaro B G, et al. Transposon mutagenesis of baculoviruses: Analysis of Trichoplusia ni transposon IFP2 insertions within the FP-locus of nuclear polyhedrosis viruses. J Virology, 1989,172(1):156～169.
5 Check E. Gene therapy put on hold as third child develops cancer. J Nature, 2005,433(7026):561.
6 Coates C J, Kaminski J M, Summers J B, et al. Site-directed genome modification: Derivatives of DNA-modifying enzymes as targeting tools. J Trends Biotechnol,2005,23(8):407～419.
7 Collier L S, Carlson C M, Ravimohan S, et al. Cancer gene discovery in solid tumors using transposonbased somatic mutagenesis in the mouse. J Nature,2005,436(7048):272～276.
8 Di Matteo M, Mátrai J, Belay E, et al. PiggyBac toolbox. J Methods Mol Biol,2012,859:241～254.
9 Ding S, Wu X, Li G, et al. Efficient transposition of the piggyBac(PB) transposon in mammalian cells and mice. J Cell,2005,122(3):473～483.
10 Dupuy A J, Akagi K, Largaespada D A, et al. Mammalian mutagenesis using a highly mobile somatic Sleeping Beauty transposon system. J Nature,2005,436(7048):221～226.
11 Dupuy A J, Jenkins N A, Copeland N G. Sleeping Beauty: A novel cancer gene discovery tool. J Hum Mol Genet, 2006,15(1):R75～R79.
12 Elick T A, Bauser C A, Fraser M J. Excision of the piggyBac transposable element in vitro is a precise even that is enhanced by the expression of its encoded transposase. J Genetica,1996,98(1):33～41.
13 Fraser M J, Cary L, Boonvisudhi K, et al. Assay for movement of Lepidopteran transposon IFP2 in insect cells using a baculovirus genome as a target DNA. J Virology,1995,211(2):397～407.
14 Fraser M J, Ciszczon T, Elick T, et al. Precise excision of TTAA-specific lepidopteran transposons piggyBac (IFP2) and tagalong (TFP3) from the baculovirus genome in cell lines from two species of Lepidoptera. J Insect Mol Biol, 1996,5(2):141～151.
15 Geurts A M, Collier L S, Geurts J L, et al. Gene mutations and genomic rearrangements in the mouse as a result of transposon mobilization from chromosomal concatemers. J PLoS Genet,2006,2(9):e156.
16 Hacker U, Nystedt S, Barmchi M P, et al. PiggyBac-based insertional mutagenesis in the presence of stably integrated P elements in Drosophila. J Proc Natl Acad Sci,2003,100(13):7720～7725.
17 Handler A M, McCombs S D, Fraser M J,et al. The lepidopteran transposon vector, piggyBac, mediates germ-line transformation in the Mediterranean fruit fly. J Proc Natl Acad Sci U S A,1998,95(13):7520～7525.
18 Huang Y, Pettitt S J, Guo G, et al. Isolation of homozygous mutant mouse embryonic stem cells using a dual selection system. J Nucleic Acids Res,2012,40(3):e21.
19 Ivics Z, Hackett P B, Plasterk R H, et al. Molecular reconstruction of Sleeping Beauty, a Tcl-like transposon from fish, and its transposition in human cells. J Cell,1997,91(4):501～510.
20 Izsvák Z, Stüwe E E, Fiedler D, et al. Healing the wounds inflicted by Sleeping Beauty transposition by double-strand break repair in mammalian somatic cells. J Mol Cell,2004,13(2):279～290.
21 Kazazian H H Jr. Mobile elements: Drivers of genome evolution. J Science,2004,303(5664):1626～1632.
22 Landrette S F, Cornett J C, Ni T K, et al. PiggyBac transposon somatic mutagenesis with an activated reporter and tracker (PB-SMART) for genetic screens in mice. J PLoS One,2011,6(10):e26650.
23 Li X, Harrell R A, Handler A M, et al. PiggyBac internal sequences are necessary for efficient transformation of target genomes. J Insect Mol Biol,2005,14(1):17～30.
24 Liu G, Aronovich E L, Cui Z, et al. Excision of Sleeping Beauty transposons:Parameters and applications to gene therapy. J Gene Med,2004,6(5):574～583.
25 Lobo N, Li X, Fraser M J Jr. Transposition of the piggyBac element in embryos of Drosophila melanogaster, Aedes aegypti and Trichoplusia ni. J Mol Gen Genet,1999,261(4～5):803～810.
26 Maragathavally K J, Kaminski J M, Coates C J. Chimeric Mos1 and piggyBac transposases result in site-directed integration. J FASEB J,2006,20(11):1880～1882.
27 Mattis A N, Willenbring H. A ZFN/piggyBac step closer to autologous liver cell therapy. J Hepatology,2012,55(6):2033～2035.
28 Meir Y J, Wu S C. Transposon-based vector systems for gene therapy clinical trials: Challenges and considerations. J Chang Gung Med J,2011,34(6):565～579.
29 Miskey C, Izsvák Z, Kawakami K. DNA transposons in vertebrate functional genomics. J Cell Mol Life Sci,2005,62(6):629～641.
30 Mitra R, Fain-Thornton J, Craig N L. PiggyBac can bypass DNA synthesis during cut and paste transposition. J EMBO J,2008,27(7):1097～1109.
31 Owens J B, Urschitz J, Stoytchev I, et al. Chimeric piggyBac transposases for genomic targeting in human cells. J Nucleic Acids Res,2012,40(14):6978～6991.
32 Sun L V, Jin K, Liu Y, et al. PBmice: An integrated database system of piggyBack (PB) insertional mutations and their characterizations in mice. J Nucleic Acids Res,2008,36(Database issue):D729～D734.
33 Thibault S T, Singer M A, Miyazaki W Y,et al. A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. J Nature Genetics,2004,36(3):283～287.
34 Wang W, Bradley A, Huang Y. A piggyBac transposon-based genome-wide library of insertionally mutated Blm-deficient murine ES cells. J Genome Res,2009,19(4):667～673.
35 Wang W, Lin C, Lu D, et al. Chromosomal transposition of piggyBac in mouse embryonic stem cells. J Proc Natl Acad Sci USA,2008,105(27):9290～9295.
36 Wernig M, Lengner C J, Hanna J, et al. A drug-inducible transgenic system for direct reprogramming of multiple somatic cell types. J Nat Biotechnol,2008,26(8):916～924.
37 Wilson M H, Coates C J, George A L Jr. PiggyBac transposon-mediated gene transfer in human cells. J Mol Ther,2007,15(1):139～145.
38 Wilson M H, Kaminski J M, George A L Jr. Functional zinc finger/Sleeping Beauty transposase chimeras exhibit attenuated overproduction inhibition. J FEBS Lett,2005,579(27):6205～6209.
39 Woltjen K, Michael I P, Mohseni P, et al.PiggyBac transposition reprograms fibroblasts to induced pluripotent stem cells. J Nature,2009,458(7239):766～770.
40 Wu S C, Meir Y J, Coates C J, et al. PiggyBac is a flexible and highly active transposon as compared to Sleeping Beauty, Tol2, and Mos1 in mammalian cells. J Proc Natl Acad Sci USA,2006,103(41):15008～15013.
41 Wu S, Ying G, Wu Q, et al. Toward simpler and faster genome-wide mutagenesis in mice. J Nat Genet,2007,39(7):922～930.
42 Yant S R, Kay M A. Nonhomologous-end-joining factors regulate DNA repair fidelity during Sleeping Beauty element transposition in mammalian cells. J Mol Cell Biol,2003,23(23):8505～8518.
43 Yant S R, Wu X, Huang Y, et al. High-resolution genome-wide mapping of transposon integration in mammals. J Mol Cell Biol,2005,25(6):2085～2094.
44 Zou C, Chou B K, Dowey S N, et al. Efficient derivation and genetic modifications of human pluripotent stem cells on engineered human feeder cell lines. J Stem Cells Dev,2012,21(12):2298～2311.

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Application of PiggyBac Transposon in Mammals

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