Abstract: To study the correlation between 50% tissue-culture infective dose (TCID₅₀) value and p27 antigen's S/P value of subgroup B avian leukosis virus and discuss their significance, chicken embryo fibroblast (CEF) cells were inoculated with subgroup B avian leukosis virus strain SDAU09C2 and samples were taken continuously for ten days after changing maintenance media. The correlations between TCID₅₀ value and p27 antigen's S/P value of ten days were then detected. Simultaneously, DF-1 cells were inoculated with it and passed to 20 generations. Samples taken from 1st, 5th, 10th, 15th and 20th generation were received the TCID₅₀ titer determination and the p27 antigen examination separately. A significant Pearson correlation was found between them in CEF cells (r=0.85277; P<0.0001) and in DF-1 cells (r=0.93000; P=0.0220). This study provided an important parameter for predicting TCID₅₀ by detecting the p27 antigen's S/P value.

Key words: subgroup B avian leukosis virus; TCID₅₀; p27 antigen; Pearson correlationMYCBiol,2012,Epub ahead of print)