阪崎肠杆菌单克隆抗体制备及ELISA检测方法的建立

Abstract

Abstract: The assay was aimed to prepare the monoclonal antibody against Enterobacter sakazakii and establish the ELISA detection method. The splenocytes from immunized mice were fused with myeloma cells SP2/0, and hybridoma cells were screened by indirect ELISA.Then culturing more monoclonal antibodies, ascites titers were identified with indirect ELISA.Subclass was determinated by mouse isotype. Double-antibody sandwich ELISA detection method was established by using the antibodies. Three hybridoma cell strains that could secret monoclonal antibodies against Enterobacter sakazakii stably were obtained,named as 5C10, 2B6 and Ab02, paired 5C10 as coating antibody and Ab02 as HRP-secondary antibody, to establish ELISA detection method. We compared with fluorescence PCR method to test 20 portion imports of milk powder, ELISA detection method had the same consequence. The monoclonal antibody against Enterobacter sakazakii and ELISA detection method had been successfully established, which laid the foundation for rapid detection of Enterobacter sakazakii in larger number.

Key words: Enterobacter sakazakii; monoclonal antibody; hybridoma cell; ELISA detection

CLC Number:

S852.4^＋3

WANG Lin, LI Meng-wei, XING You-shang, BAI Ya-duo, YIN Yi, PU Jing, QIAO Cai-xia, GAO Zhi-qiang, WEI Xue-liang, LI Chao-ming, LI Qin. Preparation of Monoclonal Antibody against Enterobacter sakazakii and Establishment of an ELISA Detection Method[J]. , 2013, 40(12): 52-55.

References

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Preparation of Monoclonal Antibody against Enterobacter sakazakii and Establishment of an ELISA Detection Method

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