猪细小病毒BQ-C毒株VP2基因的鉴定及表达

Abstract

Abstract: The BQ-C isolate of porcine parvovirus (PPV) was propagated on PK15 cell, and identified by polymerase chain reaction (PCR), haemagglutination test (HA) and electron microscope. To express the VP2 structural protein of PPV BQ-C strain, the VP2 gene was cloned into pET-30a(+) and expressed in E.coli BL21(DE3). SDS-PAGE analysis showed that the recombinant protein was about 71.5 ku. Western blotting assay confirmed that the protein was able to specifically react with PPV positive serum. In this study, the BQ-C isolate of PPV was successfully obtained, and the recombinant VP2 protein could be used for the diagnosis of PPV and vaccine development.

Key words: porcine parvovirus; recombinant VP2 protein; prokaryotic expression; immunoreactivity

CLC Number:

Q786

LIU Zhao-xia, LIU Yong-gang, BI Ke-dong, ZHOU Shun, CUI Shang-jin. Identification and Expression of VP2 Gene of Porcine Parvovirus BQ-C Isolate[J]. , 2013, 40(11): 50-53.

References

1 田莉莉,李林.猪细小病毒VP2 蛋白的截短表达与间接ELISA诊断方法的建立[J].畜牧与兽医,2011,43(11):59~64.
2 刘艳华,范伟兴,王锡乐.猪细小病毒的研究概况[J].中国畜牧兽医,2004,31(12):45~48.
3 吕建强,陈焕春,赵俊龙,等. 猪细小病毒VP2蛋白在昆虫细胞中的表达及其特性[J].微生物学报,2002,42(6):675~679.
4 李斐,曹瑞兵,周斌,等.猪细小病毒VP2蛋白的主要抗原表位基因的原核表达及检测应用[J].中国病毒学,2005,20(5):507~510.
5 殷震,刘景华.动物病毒学[M].北京:科学出版社,1997.
6 戚亭,崔尚金,张超范.猪细小病毒LXB-PPV-20-06株VP2蛋白原核表达载体的构建与表达[A]. 金宁一.第三届猪病防控学术研讨会会议论文集[C].大连:中国畜牧兽医学会,2008.
7 潘雪珠.猪细小病毒S-1株的分离和鉴定[J].上海畜牧兽医通讯,1983(1):1~3.
8 焦茂兴,郭春和,黄毓茂. 猪细小病毒结构蛋白VP2及其疫苗的研究进展[J].中国畜牧兽医,2011,38(10):231~234.
9 魏春华,刘建奎,戴爱玲,等. 猪细小病毒的分离与鉴定[J].中国畜牧兽医,2012,39(10):211~213.
10 Cartwright S F,Huck R A.Virus isolation is associated with herd infertility, abortion and stillbirth in pigs[J].Vet Rec, 1967,81:196~197.
11 Martinez C,Dalsgaard K,Lopez de Turiso J A, et al.Production empty capsids with high immunogenic activity[J].Vaccine,1992, 10(10):684~690.
12 Molitor T W,JooH S,Collett M S,et al.Porcine parvovirus: Virus purification and structural and antigenic properties of viron polypeptides[J].J Virol,1983,45:842~845.
13 Rose N,Eveno E,Grasland B,et al.Individual risk factors for post-weaning multisystemic wasting syndrome (PMWS) in pigs:A hierarchical Bayesian survival analysis[J].Prev Vet Med,2009,90(3~4):168~179.
14 Sharma R,Saikumar G.Porcine parvovirus and porcine circovirus 2 associated reproductive failure and neonatal mortality in crossbred Indian pigs[J].Trop Anim Health Prod,2010,42(3):515~522.

[1]	JIAO Linlin, CHENG Yuting, WU Qingguo, WU Shuang, WU Zhi, ZHU Shanyuan, QIAN Yingjuan. Prokaryotic Expression and Polyclonal Antibody Preparation of Duck Tembusu Virus Capsid Protein [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2395-2402.
[2]	LI Can, LI Chuchu, ZENG Wei, ZHANG Ning, JI Chunxiao, CHEN Tao. Recombinant Expression and Function Study of RegⅢγ Protein in Porcine [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2414-2426.
[3]	SONG Yue, SU Shengjie, ZHANG Fan, BAI Fan, DAI Lingli, WANG Na, WANG Dawei, YANG Qianwen, ZHAO Shihua, ZHANG Yuemei. Prokaryotic Expression and Polyclonal Antibody Preparation of Mannheimia haemolytica Truncated Leukotoxin [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2479-2486.
[4]	OU Yunwen, PAN Qin, WANG Yang, DAI Junfei, REN Shaoke, ZHANG Yang, ZHANG Jie. Truncated Expression of ORF2 Gene of Porcine Parvovirus Type 6 and Preparation of Its Polyclonal Antibody [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2487-2495.
[5]	ZHANG Fangyuan, LIN Yating, YANG Dawei, CHEN Hu, LI Guimei, SHAN Hu. Prokaryotic Expression of African Swine Fever Virus P30 Protein and Establishment of Indirect ELISA Detection Method [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2012-2022.
[6]	HU Leyu, HU Xinyao, LI Ying, ZHAO Shengmiao, SHEN Fengzhen, WANG Changfa, LI Liangliang, WANG Tongtong, REN Huiying. Bioinformatics Analysis of HO-1 Gene and Preparation of Polyclonal Antibody in Dezhou Donkey [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1499-1510.
[7]	WANG Wenjie, RU Penghui, YU Chuan, DU Fuxi, CHEN Songbiao, SHANG Ke, ZHANG Chunjie, CHENG Xiangchao. Prokaryotic Expression of VP2 Protein of Luoyang Feline Panleukopenia Virus Isolate and Polyclonal Antibody Preparation [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1511-1521.
[8]	LIU Jiaxing, HAN Xueying, ZHANG Xinru, DENG Jiaxin, YAO Lunguang, LIU Yangkun. Preparation and Immunogenicity Analysis of Ferritin Nanoparticles Carrying Antigenic Epitopes of Porcine Epidemic Diarrhea Virus [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1567-1574.
[9]	JIA Xinyue, MA Jing, ZHANG Yanyan, MA Xun, WANG Zhengrong, BO Xinwen. Bioinformatics Analysis and Prokaryotic Expression of EGR-07243 and EGR-07243 Genes of Echinococcus granulosus [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 847-858.
[10]	ZHANG Zhitao, LI Xianglong, KONG Lingli, LUO Yuxin, WANG Dongying. Prokaryotic Expression of Serine/Threonine Protein Phosphatase 2A in Fasciola gigantica and Expression Analysis at Different Stages [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1107-1117.
[11]	GAN Lu, ZHENG Huizhen, NUO Mingdalai, LIU Yan, JIN Min, HE Wenwen, WEN Licui, LI Yongchang, GAILIKE·Bayinchahan. Propagation of Trypanosoma evansi Yili Strain and Cloning, Expression and Bioinformatics Analysis of Its PFR Gene [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 469-478.
[12]	LYU Shuangfei, MA Xun, WANG Jing, KONG Cuilian, KOU Lijun, LIU Caixia, SHI Weidi, KANG Lichao, REN Huijie. Prokaryotic Expression and Immunoreactivity of PA-LF1 Fusion Protein of Bacillus anthracis [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 674-683.
[13]	ZHANG Kai, ZHANG Chunping, GE Shengqiang, SUN Chunxi, CHENG Jie, FU Chunyu, WANG Gang, NIU Xing, PENG Jun. Expression of p22 Protein of African Swine Fever Virus,Preparation of Polyclonal Antibody and Establishment of Indirect ELISA Method [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 270-279.
[14]	ZHU Lilin, WANG Houkun, CHEN Xueyang, LIU Jing, LIANG Xiongyan, FANG Chun, YANG Yuying. Effect of Chicken IFIT5 on Replication of J Subgroup Avian Leukemia Virus Within DF-1 Cells [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 310-316.
[15]	WANG Yuchen, TIAN Guangyuan, ZHOU Yaping, GUO Ting, ZHAO Hongmei, SUN Yajie, ZHAO Fengmiao, BIAN Yuchen, YU Jialiang, HAO Yongqing. Prokaryotic Expression of BPIV3 NP Protein and Its Immunoenhancing Effect on the BPIV3 Inactivated Vaccine [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3122-3130.

Identification and Expression of VP2 Gene of Porcine Parvovirus BQ-C Isolate

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments