2006—2012年鲁豫冀地区15株猪繁殖与呼吸综合征病毒的分离鉴定及ORF5/Nsp2基因的遗传变异分析

Abstract

Abstract: To study the genetic variation of porcine reproductive and respiratory syndrome virus(PRRSV)in Shandong, Henan and Hebei province of China, 42 samples from pig farms in three provinces were isolated and identified during 2006 to 2012. 15 PRRSV isolates were successfully isolated, identified by PCR and biological characteristics were studied. The complete ORF5 and partial Nsp2 genes of 15 isolates were amplified and sequenced. The deduced amino acid sequences were blasted with deduced sequences of 68 ORF5 genes and 40 Nsp2 genes in GenBank. The analysis showed that all 15 isolates belonged to American groups, 13 of them presented discontinuous amino acid deletions in Nsp2, and the homology of ORF5 deduced amino acid sequences with JXA1 were from 95.5% to 97.5%. Besides, isolate SDDY2007 showed higher similarity to vaccine strain RespPRRSV MLV(98.5%), which was located in America subgroupⅡ. A more interesting thing was that, though Nsp2 sequence of HN25-2009 contained 30 amino acids deletion, its deduced amino acid sequences showed higher similarity to VR2332(97.5%). In short, highly pathogenic PRRSV was the dominant PRRSV in Shandong, Henan and Hebei during 2006 to 2012. Although there were some genetic variations among PRRSV isolates in three provinces, no obvious regional characteristics could be found.

Key words: porcine reproductive and respiratory syndrome virus; isolation; genetic diversity; ORF5 gene; Nsp2 gene

CLC Number:

S852.65

TANG Na, GUAN Yu, WEI Feng, WANG Jin-liang, QU Guang-gang, XIAO Yue-qiang, LI Feng, XIE Jin-wen, ZHANG Song-lin, ZHANG Qian, YU Xin-you, SHEN Zhi-qiang, LIU Zeng-shan. Isolation, Identification and ORF5/Nsp2 Genes Genetic Diversity Analysis of 15 Porcine Reproductive and Respiratory Syndrome Virus Isolates from Shandong, Henan and Hebei Areas During 2006 to 2012[J]. , 2013, 40(10): 26-32.

References

1 王艳艳. 山东省猪繁殖与呼吸综合征、猪流感流行病学调查[D]. 泰安:山东农业大学,2010.
2 刘飞,王开功,周碧君,等. 贵州省PRRSV分离株Nsp2基因的克隆及序列分析[J]. 中国预防兽医学报,2010,32(12):924~928.
3 郎广平, 郭敏, 韩盈盈,等. 猪繁殖与呼吸综合征诊断方法与疫苗研究进展[J]. 中国畜牧兽医,2013,40(6): 195~198.
4 童武,周艳君,肖少波,等. 2010年国内部分省份猪场常见病毒性疾病的病原学调查[J].中国动物传染病学报,2011,5:1~7.
5 Darwich L, Da I, Mateu E. Certainties, doubts and hypotheses in porcine reproductive and respiratory syndrome virus immunobiology[J]. Virus Research, 2010, 154: 123~130.
6 Dokland T. The structural biology of PRRSV[J].Vrius Research, 2010,154:86~97.
7 Johnson C R, Griggs T F, Gnanandarajah J, et al. Novel structural protein in porcine reproductive and respiratory syndrome virus encoded by an alternative ORF5 present in all arteriviruses[J]. J Gene Virol, 2011, 92: 1107~1116.
8 Kim W I, Kim J J, Cha S H, et al. Significance of genetic variation of PRRSV ORF5 in virus neutralization and molecular determinants corresponding to cross neutralization among PRRS viruses[J].Veterinary Microbiology, 2013,162(1):10~22.
9 Liu D, Zhou R, Zhang J L, et al. Recombination analyses between two strains of porcine reproductive and respiratory syndrome virus in vivo[J]. Virus Research, 2011,155(2): 473~486.
10 Martin-Valls G E, Burgara-Estrella A J, Darwich L, et al. Ecombination events are common in genotype 1 and 2 PRRS virus worldwide and may generate mosaic viruses[A]. 2013 International PRRS symposium[C].2013.
11 Murtaugh M P, Stadejek T, Abrahante J E, et al. The ever-expanding diversity of porcine reproductive and respiratory syndrome virus[J]. Virus Research, 2010, 154: 18~30.
12 Rosendal T. Association between PRRSV ORF5 genetic distance and differences in space, time, ownership, and animal sources among commercial pig herds[A]. 2011 International PRRS Symposium, Chicago[C]. 2011.
13 Wang F X, Song N, Chen L Z, et al. Non-structural protein 2 of the porcine reproductive and respiratory syndrome (PRRS) virus: A crucial protein in viral pathogenesis, immunity and diagnosis[J]. Research in Veterinary Science, 2013, 95(1):1~7.
14 Xu X G, Wang Z S, Zhang Q, et al. Baculovirus as a PRRSV and PCV2 bivalent vaccine vector: Baculovirus virions displaying simultaneously GP5 glycoprotein of PRRSV and capsid protein of PCV2[J]. J Virol Methods, 2012, 179: 359~366.
15 Zhou Z, Ni J Q, Cao Z, et al. The epidemic status and genetic diversity of 14 highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) isolates from China in 2009[J]. Vet Microbiol, 2011, 150:257~269.

[1]	LU Yukui, LIU Jiaqi, ZHONG Jiacheng, CHEN Jidang, ZHU Wanjun, ZHANG Yishan, ZHANG Jipei. Isolation,Identification and Pathogenicity Analysis of Avibacterium paragallinarum from Geese [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2562-2572.
[2]	KANG Pu, REN Zhaowen, CAI Rujian, LYU Zongji, HUANG Yuhao, WANG Xiaohu. Variation Regularity and Transmission of PRRSV-2 Lineage 8 Strains in Guangdong and Surrounding Provinces [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2001-2011.
[3]	CHEN Tianbao, WANG Yani, LIU Baoling, WANG Gang, WANG Xiaohu, LIU Xuhui, CAI Rujian, HE Dongsheng. Research Progress on the Anti-Porcine Reproductive and Respiratory Syndrome Virus Effect and Mechanism of Traditional Chinese Medicine [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2114-2129.
[4]	LI Zhijie, ZHAO Ying, MA Xin, WANG Meng, YU Sijiu, WANG Libin, PAN Yangyang. Research Progress on Isolation Methods and Main Reproductive Functions of Exosomes from Animal Follicular Fluid [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1480-1488.
[5]	TIAN Changqing, ZHANG Kunzhong, QI Yumei, SHI Wenjing, DONG Zhijie, ZHANG Haohao, HE Zengwen, ZHI Ji, ZHAO Xuehui, CHONG Qian, XUE Huiwen, GOU Huitian. Isolation,Identification and Biological Characteristics Analysis of a Strain of Listeria monocytogenes from Environment [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1543-1555.
[6]	CAO Yingying, LIANG Liang, ZENG Yirong, ZHONG Qinghua, YUAN Xiaofang, TANG Haibo, LENG Jing. Isolation,Identification and Biological Characteristics of Salmonella from Tupaia belangeri chinensis [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1718-1728.
[7]	LIU Kexin, WANG Chao, ZHANG Ao, LIU Jiali, TAN Bin, ZHANG Shuqin. Isolation,Identification and Genome Sequence Analysis of Bovine Viral Diarrhea Virus LN-1 Strain [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1150-1159.
[8]	LI Huawei, GUO Kewei, WANG Xuying, ZHANG Xiaoyu, LU Ziwei, LI Xinfeng, MA Hui, HOU Wenjing. Eukaryotic Expression and Subcellular Localization of Nonstructural Protein 7 of Porcine Reproductive and Respiratory Syndrome Virus [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1160-1168.
[9]	CHEN Dong, ZHANG Yanhao, ZHANG Yang, LI Sen, ZHOU Zutao, LI Zili. Isolation,Identification and Drug Sensitivity of 3 Strains of Riemerella anatipestifer [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1250-1258.
[10]	WANG Bingyi, MA Hongcai, ZOU Minghao, FAN Shijie, YUAN Zhenjie, Labaciren, DONG Hailong, ZENG Jiangyong. Isolation,Identification and Drug Resistance Analysis of Streptococcus from Yak [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 745-753.
[11]	ZHONG Huachen, WANG Lifang, GUO Chenyang, LIU Jialin, SONG Jie. Isolation,Identification and Drug Resistance Analysis of Pathogenic Bacteria from Milk Samples and Environment of Mastitis in Inner Mongolia [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 817-826.
[12]	SUN Yu, ZHANG Hao, LI Changyang, TAO Yanzi, WANG Qiuju. Isolation,Identification and Biological Characteristics of Antioxidant Bacillus from Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 359-367.
[13]	WEI Jin, WANG Jia, ZHAO Haoran, LIU Bo, LIU Yuanjie, YAO Wensheng, LIU Yan, MA Xin. Isolation, Identification and Antimicrobial Susceptibility of Mycoplasma gallisepticum from Different Areas [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 368-376.
[14]	YANG Fumei, YANG Linfu, ZENG Cheng, YANG Zhi, WANG Kang, DUAN Gang, DAI Feiyan. Isolation,Identification and Biological Characterization Analysis of a Strain of Carnobacterium maltaromaticum from Barking Deer [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 398-407.
[15]	ZHANG Wei, WU Hua, YIN Yajie, LI Songli, HOU Shaohua. Isolation and Identification of Novel Duck Reovirus and Its σC Gene Sequence Analysis [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3520-3529.

Isolation, Identification and ORF5/Nsp2 Genes Genetic Diversity Analysis of 15 Porcine Reproductive and Respiratory Syndrome Virus Isolates from Shandong, Henan and Hebei Areas During 2006 to 2012

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments