›› 2013, Vol. 40 ›› Issue (10): 206-210.

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Isolation, Identification and Genetic Evolution Analysis of F Gene of Newcastle Disease Virus TH-1 from Duck

DAWA Zhuo-ma1, JIN Hong-yan1, FENG Jia-wang1, YANG Kang-lin1, JIANG Yi-tong2, LV Jian3, HOU Shao-hua2   

  1. 1. Tibet Vocational Technical College, Lhasa 850030, China;
    2. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
    3. Patent Examination Cooperation Center of the Patent Office, Beijing 100193, China
  • Received:2013-05-21 Online:2013-10-20 Published:2013-12-19

Abstract: A Newcastle disease virus (NDV) was isolated from the death duck in Jilin province Tonghua city, and TH-1 strain was named. It was identified with hemagglutination test, hemagglutination inhibition test,neutralization test, PCR and sequence analysis. It was proved virulent because of MDT of 53.4 h, 1.85 of ICPI,and 2.575 of IVPI. The cleavage site of the virus F protein was 112-RRQKRF-117, according to highly pathogenic strain character. The homology of F gene was 99.3% with that of strain mallard China HLJ, separated from wild duck. By analyzing genotype and homology of TH-1 and other NDV strains, it showed that TH-1 strain was belonged to genotype Ⅶ, which was the dominated genetype in China in recently.

Key words: Newcastle disease virus; isolation; identification; F gene; sequence analysis