Abstract: 73 Staphylococcus strains including 50 Staphylococcus aureus (S.aureus) and 23 coagulase-negative Staphylococci were isolated from pork and milk samples with collected from Guizhou province in 2010. Antimicrobial susceptibility testing was performed with agar dilution method according to the recommendations of the Clinical and Laboratory Standards Institute (CLSI). Statistical methods were used to analyze the differences of antimicrobial susceptibility between S.aureus and coagulase-negative Staphylococci which isolated from different samples. The results showed that these isolates displayed a high degree of resistance to the common antimicrobials and showed multi-drug resistance. Moreover, isolates which isolated from the milk samples showed much higher multi-drug resistance than those isolated from the pork samples. S.aureus which isolated from the pork samples demonstrated primarily resistant to penicillins,tetracyclines and macrolides,while S.aureus which isolated from the milk samples showed primarily resistant to penicillins,cephalosporins, macrolides, tetracyclines, clindamycin, tiamulin and rifampicin. The coagulase-negative Staphylococci which isolated from the pork samples displayed primarily resistant to ampicillin,oxacillin and tiamulin, and the resistance profile of coagulase-negative Staphylococci from milk samples showed no remarkable difference from that of the S.aureus isolated from the milk samples.

Key words: Staphylococcus aureus; coagulase-negative Staphylococci; resistance; pork; milk