喹喔啉-2-羧酸人工抗原合成与抗体制备

Abstract

Abstract: Quinoxaline-2-carboxylic acid (QCA) and γ-amino butyric acid derivative of QCA (QCA-ABA) were conjugated to bovine serum albumin (BSA). The conjugates QCA-BSA, QCA-ABA-BSA used to immunize New Zealand White rabbits were identified with UV scanning spectrum and the specificity of antibodies were detected by indirect ELISA and indirect compete ELISA. The antibodies produced with QCA-BSA, QCA-ABA-BSA conjugates showed the titers of 12.8×10⁴, 6.4×10⁴, respectively. These antibodies were not specific for the QCA moiety, IC₅₀ values of >10000 ng/mL, but specific for the QCA derivatization (QCA-ABA), IC₅₀ values of 14.2 and 7.75 ng/mL, respectively, and cross-reactivity of 12% with 3-methyquinoxaline-2-carboxylic acid, 0.038% with carbadox and 0.028% with cyadox, but did not cross-react with other veterinary drugs. The study laid a solid foundation for rapid detection of QCA in edible animal tissues.

Key words: quinoxaline-2-carboxylic acid; carbadox; hapten; artificial antigen; antibody

CLC Number:

S859.84

ZHANG Ze-ying, YUAN Zong-hui. Antigens Synthesis and Antibodies Preparation of Quinoxaline-2-Carboxylic Acid[J]. , 2013, 40(10): 152-155.

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Antigens Synthesis and Antibodies Preparation of Quinoxaline-2-Carboxylic Acid

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