赤狐黑色素皮质激素受体-1基因结构特征预测及分子进化分析

Abstract

Abstract: In order to investigate the molecular characterization of melanocortin-1 receptor (MC1R) gene, the base composition and structure characteristics of red fox MC1R gene registered in GenBank were predicted and analyzed by the method of bioinformatics. In addition, the phylogenetic tree of MC1R protein was constructed by means of MEGA 5.05 software between 12 species. The results showed that the length of red fox MC1R gene coding sequence was 954 bp, which encoded 317 amino acids. The content of G+C was higher than that of A+T. The estimated molecular weight of MC1R protein in red fox was 34.8694 ku, with an isoelectric point of 9.13, belonged to the stable alkalinous protein with hydrophily. The MC1R protein of red fox contained 7 obvious strong transmembrane regions, 7 phosphorylation sites and the 157-threonine amino acid site could be phosphorylated by PKC. The main secondary structure of MC1R protein was α-helix, and it was a member of G protein coupled receptor family, contained a number of functional motifs. The similarity comparison and phylogenetic tree indicated that the evolutionary distance of red fox MC1R protein was the most homogeneous to arctic fox, dog and raccoon dog.

Key words: Vulpes vulpes; MC1R gene; structural characteristics; bioinformatics

CLC Number:

Q754

SONG Xing-chao, YANG Fu-he, BA Heng-xing, XU Chao, BAO Kun, XING Xiu-mei, WEI Hai-jun. Prediction on Structural Characteristics and Phylogenetic Evolution Analysis of MC1R Gene in Red Fox(Vulpes vulpes)[J]. , 2013, 40(1): 9-13.

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Prediction on Structural Characteristics and Phylogenetic Evolution Analysis of MC1R Gene in Red Fox(Vulpes vulpes)

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