基于重组NS1蛋白猪细小病毒野毒抗体间接ELISA诊断方法的建立与应用

Abstract

Abstract: Using recombinant NS1 protein as coating antigen, an indirect ELISA was successfully developed to detect wild virus antibody to PPV. The results of detecting the positive sera of other five swine diseases(CSFV, PRRSV, PRV, PCV2, PEDV) using the method were negative. Coefficient of variability percent (C.V%) of intro-batch and inter-batch duplicative tests was less than 5% and 10%, respectively. Lowest antibody titer of positive control serum was 1:12800. Comparing with the HI assay, the concordance rate was 100%. Therefore, the NS1-ELISA had good specificity, sensitivity and repeatability, and could be used as a simple and rapid serology detection method for monitoring the anti-PPV wild virus antibody and epidemiologic survey of PPV.

Key words: porcine parvovirus; NS1 protein; indirect ELISA; diagnosis

CLC Number:

Q786

TANG Zhong-he. Establishment and Application of an Indirect ELISA for Detecting Wild Virus Antibodies to Porcine Parvovirus using NS1 Recombinant Protein[J]. , 2013, 40(1): 46-49.

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Establishment and Application of an Indirect ELISA for Detecting Wild Virus Antibodies to Porcine Parvovirus using NS1 Recombinant Protein

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