奶牛瘤胃液中浸麻类芽孢杆菌的分离鉴定

›› 2013, Vol. ›› Issue (6): 246-249.

Isolation and Identification of Paenibacillus macerans Strain from Cows Rumen Fluid

DENG Hui-yuan^1,2, LI Zhi-zhong², ZHANG Kai¹, LI Jian-xi¹

1. Institute of Lanzhou Animal Science and Veterinary Pharmaceutics, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China;
2. School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China

Received:2012-10-30 Online:2013-06-20 Published:2013-06-20

Abstract

Abstract: We used flat crossed separation method to get a bacterial Y-1, which was isolated from a cow rumen fluid. Simulating rumen environment in vitro to proliferate Y-1. The test adopted the methods, which include morphological observation, biochemical reactions and 16S rDNA gene homology comparison, and constructed the phylogenetic tree for Y-1.The results indicated that Y-1 was Paenibacillus macerans strain, and analyzed the reasons for the presence of bacteria in the rumen fluid.

Key words: Paenibacillus macerans; morphological identification; biochemical tube identification; 16S rDNA sequcing

CLC Number:

S823

DENG Hui-yuan, LI Zhi-zhong, ZHANG Kai, LI Jian-xi. Isolation and Identification of Paenibacillus macerans Strain from Cows Rumen Fluid[J]. , 2013, (6): 246-249.

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Isolation and Identification of Paenibacillus macerans Strain from Cows Rumen Fluid

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