高灵敏度酶联免疫法快速检测动物源性产品中氯霉素残留的研究

Abstract

Abstract: In this study, an ELISA method for determination of chloramphenicol (CAP) residue in animal products was used. According to the instruction of ELISA kit, CAP from pretreatment samples which was competed with CAP-HRP, and could be combined antibody coated. The content of CAP could be measured by color produced by substrate solution and HRP. The limit of the detection method in animal products was less than 0.1 μg/kg, and recoveries ranged from 74.4% to 105.4% for fortified samples at levels of 20 to 300 ng/kg with coefficient of variation values less than 15%.The ELISA kit could meet the requirement of detecting CAP residue in animal products.

Key words: chloramphenicol; ELISA; animal products; residue

CLC Number:

S852.4

CHEN Xia, YANG Xiao-xia, LUO Peng-hui, LUO Peng-jie. Study on Rapid Determination of Chloramphenicol Residue in Animal Products by High Sensitive ELISA[J]. , 2013, (6): 233-236.

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Study on Rapid Determination of Chloramphenicol Residue in Animal Products by High Sensitive ELISA

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