Abstract: A rapid method for determination of phenylethanolamine A in formula feed by using ultra performance liquid chromatography (UPLC)-PDA had been developed.Phenylethanolamine A in formula feed was extracted with methanol containing 0.1% formic acid,followed mix cation ionic exchange solid phase extraction cartridge clean up,through the sample matrix correction external standard got quantitative.In the optimized conditions,a good linearity of phenylethanolamine A in feed sample matrix was obtained at the concentrations from 0.1 to 20.0 mg/kg,the linear relative coefficient of calibration curve (R²) was 0.9945.The recovery of phenylethanolamine A spiked in formula feed at different concentrations was above 90.36%,and the coefficient of variation was below 5.98%.The limit of detection was 0.06 mg/kg and the limit of quantification was 0.10 mg/kg. The method provided advantages of high sensitive,good precision,reliable data,easy operation and the potential use for rapid determination of phenylethanolamine A for supervising formula feed.

Key words: ultra performance liquid chromatography; phenylethanolamine A; feed