Abstract: A virus strain isolated from young geese (gosling)with fever, feed uptake decline, diarrhea, standing instability and dyskinesia signs, in Guangdong area. The isolated virus ELD₅₀ to muscovry duck was 10^-2.68/0.2 mL, and had no hemagglutination activity on chichen,duck,goose or pigeon sera at pH 7.2. A animal regression experiment was conducted, and a similar disease was reproduced in 15-day healthy geese. Using specific PCR primer pairs,which was used to amplified partial E gene of the BYD virus, a 401 bp nucleotide fragment was obtained by RT-PCR.The obtained sequence was compared to the GenBank database using BLAST and the closest sequences were BYD virus(GenBank accession JF312912), which caused duck egg-drop syndrome in China, and a novel Flavivirus strain JS804(GenBank accession JF895923), which isolated from geese in Jiangsu China, with a similarity of 99%. These results suggested that the isolated virus from geese in Guangdong was the BYD virus of Flavivirus genus and called a brief name for BYD-GD1.

Key words: Flavivirus strain; geese; isolation; identification