| 1 卢圣忠.牛和猪精子注射法受精研究.北京:中国农业科学院,1992.2 李俊,田永祥,曹斌云,等. 套皿法在猪卵母细胞体外成熟培养中的应用[J].湖北农业科学,2004(6):74~76.3 苗向阳,方昌阁,邵建军,等. 单精注射法生产转GFP基因猪胚胎的研究[J]. 中国畜牧兽医,2007,34(6):44~46.4 苟克勉,安晓荣,田见晖,等.绵羊胞内单精子注射技术[J].实验生物学报,2002,35:103~108.5 桑润滋.动物繁殖生物技术[M].第2版.北京:中国农业出版社,2006.6 Funahashi H, Day B N. Effects of different serum supplements in maturation medium on exotic and cytoplasm maturation of pig oocytes[J].Therigenology,1993,39:965~973.7 Goud P T,Good A P,Qian C, et al. In vitro maturation of human germinal vesicle stage oocytes: Role of cumulus cells and epidermal growth factor in the culture medium[J]. Hum Reprod,1998, 13(6): 1638~1644.8 Goto K.Bovine microfertilization and embryo transfer[J]. Mol Reprod Devel,1993,36:288~290.9 Kishikawa H, Tateno H, Yanagimachi R. Fertility of mouse spermatozoa retrieved from cadavers and maintained at 4 ℃[J]. Journal of Reproduction and Fertility,1999,116:217~222.10 Kuretake S, Kimura Y, Hoshi K, et al. Fertilization and development of mouse oocytes injected with isolated sperm head[J].Biology of Reproduction,1996,55:789~795.11 Kikuchi K, Izaike Y, Noguchi J, et al. Decrease of histone H1 kinase activity in relation to parthenogenetic activation of pig follicular oocytes matured and aged in vitro[J].Reprod Fertil,1995,105(2):325~330.12 Lee J W, Tian X C, Yang X Z. Failure of male pronucleus formation is the major cause of lack of fertilization and embryo development in pig oocytes subjected to intracytoplasmic sperm injection [J]. Biol Reprod, 2003, 58: 1341~1347.13 Lai L X, Sun Q Y, Wuc G M, et al. Development of porcine embryos and offspring after intracytoplasmic injection with liposome transfected or non-transfected sperm into in vitro matured oocytes[J].Zygote,2001,9:339~346.14 Merriman J A. The effect of follicle stimulation hormone and epidermal growth factor on the developmental capacity of in vitro matured mouse oocytes[J]. Hum Reprod, 1998, 13(3): 690~695.15 Probst S,Rath D. Production of piglets using intracytoplasmic sperm injection with flowcytometrically sorted boar semen and artificially activated oocytes[J]. Theriogenology,2002,8691:1~13.16 Petters R M, Wells K D. Culture of pig embryos[J]. Reprod Fertil,1993,48:61~73.17 Park C Y, Uhm S J,Song S J. Increase of ICSI efficiency with hys1uronic acid binding sperm for low aneuploidy frequency in pig[J].Theriogenology,2005,64:1158~1169.18 Suttner R, Zakhartchenko V, Stojkovic P,et al.Intracyto-plasmic sperm injection in bovine:Effects of oocyte activation, sperm pretreatment and injection technique[J].Theriogenology,2000,54:935~948.19 Yanagimachi R. Gamete manipulation for development:New methods for conception[J] .Reprod Fertil Dev,2001,13(1):3~14. |
