鹅细小病毒SS/10株的分离鉴定及生物学特性研究

Abstract

Abstract: A strain of goose parvovirus (SS/10) was isolated from gosling hepar and biological characteristics of the virus were studied.The result indicated that the ELD₅₀ to muscovy duck embryos was 10^-4.6/0.3 mL and no goslings were dead after inoculation,but pathological change by tissue section was evident. Sequence analysis showed that the homology with DY strain was lower than other reference strains in VP1,VP2 and VP3 genes respectively and there was no difference in NS1 and NS2 genes; VP3 gene belonged to a branch with 82-0321V,VG32/1 and B strains published in GenBank. It had closest genetic relationship with 82-0321V and farthest genetic relationship with muscovy duck parvovirus GD strain. Glycosylation sites analysis showed that VP3 had one less glycosylation site than B.

Key words: goose parvovirus; isolation and identification; tissue section; sequence analysis; biological characteristics

CLC Number:

S852.65⁺9.2

HU Zi-li, LIU Hai-ling, HUANG Wu, RAO Lei, LUO Kai-jian. Isolation,identification and Biological Characteristics of a Goose Parvovirus Strain SS/10[J]. .

References

1 王明俊. 兽医生物制品学[M]. 北京:中国农业出版社, 1997.

2 毛文智,邵洪泽,姚新华,等. 鹅细小病毒强毒株XKY10株的分离、鉴定及其生物学特性分析[J]. 中国畜牧兽医, 2012,39(3):171~173.

3 卡尔尼克 B W. 禽病学[M]. 北京:中国农业大学出版社, 1999.

4 李宝臣, 齐岩, 马波, 等. 鹅细小病毒不同毒株VP3基因的序列分析比较[J]. 中国预防兽医学报, 2003, 25(6):430~433.

5 李桂霞, 刘胜旺, 孔宪刚, 等. 鹅细小病毒HG5/82株的分离鉴定及生物学特性的研究[J]. 中国预防兽医学报, 2005, 27(3):196~201.

6 周斌, 苗晋锋. 苏州地区小鹅瘟的诊断分析和防治对策[J] .中国家禽, 2001, 23: 25~26.

7 殷震, 刘景华. 动物病毒学[M]. 第2版. 北京:科学出版社,1997.

8 徐镔蕊, 吕艳丽, 董卫星, 等. 蛋鸡骨髓细胞瘤病的病理学诊断[J]. 畜牧兽医学报, 2002, 33(6):562~564.

9 董国雄, 李新华, 李俊宝. 抗小鹅瘟病毒中和性单克隆抗体研制及实验防治效果[J]. 江苏农学院学报, 1996, 17(3):7~10.

10彭万强, 朱治远, 黄承锋, 等. GPV鸭胚化弱毒苗对雏鹅的免疫研究[J]. 中国兽医科技, 1992, 22(4):9~11.

11Brown K E, Green S W. Goose parvovirus An autonomous member of the dependovirus genus[J]. Virology, 1995, 210:283~291.

12Change P C, Shien L H, Wang M S, et al. Phylogentic analysis of parvovirus isolated in Taiwan from ducks and geese[J].Avian Pathology, 2000, 29:45~49.

13Gough R E. Goose parvovirus infection[J]. Disease of Poultry,1991,3:684~690.

14Shien J H, Wang Y S, Chen C H, et al. Identification of sequence changes in live attenuated goose parvovirus vaccine strains developed in Asia and Europe [J].Avian Pathol, 2008, 37(5):499~505.

15Takehara K, Nishio T, Hayashi Y, et al . An outbreak of goose parvovirus infection in Japan[J]. Vet Med Sci, 1995, 57(4):777~779.

16Tatar-Kis T, Mato T, et al. Phylogenetic analysis of Hungarian goose parvovirus isolates and vaccine strains[J]. Avian Payhology, 2004, 33(4): 438~444.

17Zadori Z R, Stefancsik T, Rauch K J. Analysis of the complete nucleotide sequences of goose and muscovy duck parvoviruses indicates commol/Lon ancestral origin with adeno-associated virus 2[J]. Virology, 1995, 212: 562~573.

[1]	LU Yukui, LIU Jiaqi, ZHONG Jiacheng, CHEN Jidang, ZHU Wanjun, ZHANG Yishan, ZHANG Jipei. Isolation,Identification and Pathogenicity Analysis of Avibacterium paragallinarum from Geese [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2562-2572.
[2]	LI Zhijie, ZHAO Ying, MA Xin, WANG Meng, YU Sijiu, WANG Libin, PAN Yangyang. Research Progress on Isolation Methods and Main Reproductive Functions of Exosomes from Animal Follicular Fluid [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1480-1488.
[3]	TIAN Changqing, ZHANG Kunzhong, QI Yumei, SHI Wenjing, DONG Zhijie, ZHANG Haohao, HE Zengwen, ZHI Ji, ZHAO Xuehui, CHONG Qian, XUE Huiwen, GOU Huitian. Isolation,Identification and Biological Characteristics Analysis of a Strain of Listeria monocytogenes from Environment [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1543-1555.
[4]	CAO Yingying, LIANG Liang, ZENG Yirong, ZHONG Qinghua, YUAN Xiaofang, TANG Haibo, LENG Jing. Isolation,Identification and Biological Characteristics of Salmonella from Tupaia belangeri chinensis [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1718-1728.
[5]	WANG Bingyi, MA Hongcai, ZOU Minghao, FAN Shijie, YUAN Zhenjie, Labaciren, DONG Hailong, ZENG Jiangyong. Isolation,Identification and Drug Resistance Analysis of Streptococcus from Yak [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 745-753.
[6]	ZHONG Huachen, WANG Lifang, GUO Chenyang, LIU Jialin, SONG Jie. Isolation,Identification and Drug Resistance Analysis of Pathogenic Bacteria from Milk Samples and Environment of Mastitis in Inner Mongolia [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 817-826.
[7]	SUN Yu, ZHANG Hao, LI Changyang, TAO Yanzi, WANG Qiuju. Isolation,Identification and Biological Characteristics of Antioxidant Bacillus from Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 359-367.
[8]	WEI Jin, WANG Jia, ZHAO Haoran, LIU Bo, LIU Yuanjie, YAO Wensheng, LIU Yan, MA Xin. Isolation, Identification and Antimicrobial Susceptibility of Mycoplasma gallisepticum from Different Areas [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 368-376.
[9]	YANG Fumei, YANG Linfu, ZENG Cheng, YANG Zhi, WANG Kang, DUAN Gang, DAI Feiyan. Isolation,Identification and Biological Characterization Analysis of a Strain of Carnobacterium maltaromaticum from Barking Deer [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 398-407.
[10]	ZHANG Wei, WU Hua, YIN Yajie, LI Songli, HOU Shaohua. Isolation and Identification of Novel Duck Reovirus and Its σC Gene Sequence Analysis [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3520-3529.
[11]	XI Jing, YI Jihai, WANG Yueli, XU Zhenyu, LI Peidong, ZHANG Jiangwei, CHEN Chuangfu. Isolation, Identification and Genotype Analysis of Group A Bovine Rotavirus G10 Strain XJ-2022 [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3530-3538.
[12]	LIU Xiaolan, LIU Changjin, YU Wenyang, LI Xiaoxiang, BIAN Yanchao, HUANG Xiaohua, LUO Feng, DENG Shunzhou. Isolation and Identification of Porcine Rotavirus Jiangxi Strain AY01 [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3151-3162.
[13]	XU Minsheng, KE Haiyi, SHI Keda, YANG Dongxia, ZHAI Shaolun, ZANG Yingan, LI Chunling. Isolation and Identification of Actinobacillus pleuropneumoniae from Guangdong Province and Detection and Analysis of Drug Resistance Phenotype and Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3212-3225.
[14]	YANG Zemin, LI Shuang, JIN Zhengyu, LIAO Yixiao, YANG Ying, WEN Ming. Isolation,Identification and Biological Characteristics of a Strain of Lactobacillus plantarum from Carp [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2805-2811.
[15]	YIN Yajie, LIANG Ruiying, CUI Huan, MENG Lijia, NI Weiling, QIAO Sina, GUO Kangkang, ZHANG Bo, LI Songli, HOU Shaohua, DONG Shishan. Isolation,Identification of Duck Tembusu Virus,and Genetic and Evolutionary Analysis of Its Envelope Protein [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1497-1506.

Isolation,identification and Biological Characteristics of a Goose Parvovirus Strain SS/10

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments