Abstract: A PCR kit for detection bovine tuberculosis IS6110 gene was developed based on PCR principle with designing a pair of primer from GenBank. A specific band (317 bp) was detected with the PCR kit. By sensitivity analysis,a minimum of 1.025 pg pathogen DNA was detected, and pathogens of control groups,such as Pseudo tuberculosis,Actnobacillus,Escherichia coli,Pastueurella,Salmonella,Staphylococcus aureus,Streptococci showed nagative.The kit could be stored at －20 ℃ for a year and having a repeating performance. With the PCR kit, 24 cases was diagnosed and the result was similar to tuberculin test. The PCR kit can be a good method for clinical diagnosis of bovine tuberculosis with rapid, sensitivity and specificity.

Key words: bovine tuberculosis; PCR kit; IS6110 gene