Abstract: The aims of this study were to amplify and analyze the sequence of internal transcribed spacers (ITS) of ribosomal DNA (rDNA)of Anisakid larvae samples isolated from frozen pacific cod imported from Japan. The DNA of the samples were extracted and the ITS sequences were amplified by PCR with primers NC5 and NC2. The products were cloned into pMD18-T vector and the inserts were successfully sequenced. The results revealed ITS inserts were 906 bp in length and consisted of partial 18S,28S and complete ITS1 (353 bp),5.8S(157 bp)and ITS2(299 bp) rDNA sequences. The similarity in ITS1 and ITS2 sequences among the Anisakid larvae sample and Pseudoterranova decipiens available in GenBank were over 99.7%,and lower homology with other nematodes.The results of the present study provided a foundation for further studies of Anisakid larvae.

Key words: Anisakid larvae; internal transcribed spacers(ITS); PCR amplification; sequence analysis