Abstract: To study the repeated superovulation effect in brief period in wagyu-catalo,100 head of wagyu-catalo were superovulated continuously for three times from Jun in 2011 to Aug in 2011. The three times superovulation obtained the mean of embryo recovered were 11.65±8.96,13.28±7.69 and 13.24±8.39,respectively;the mean of usable embryos were 7.52±7.16,8.51±6.94 and 8.92±6.59,respectively. The experimental results indicated that,a good result could be obtained by proceeding consecutively three times repeated superovulation in 33 d interval in wagyu-catalo. There were obvious individual differences in the effect of superovulation.The superovulation efficiency of primiparous was a little better than multipara cow(P>0.05),but the number of available embryos of multiparous was better than primiparous cow notablely(P<0.05).

Key words: wagyu-catalo; repeated superovulation; the mean of usable embryos