Abstract: Bacillus sublitis was inoculated in soybean meal substrate, this fermented soybean meal was detected crude protein and hydrolysis of protein. Compared the increase of crude protein and hydrolysis of protein, fermentation conditions including carbon resource, temperature, moisture, culturetime and inoculant concentration were optimized by orthogonal design. The result showed that the sequence of effect factor for crude protein increase were cornmeal ration> culture time > temperature > wheat bran ration > moisture > wheat short ration > inoculant concentration. The highest crude protein increase was 28.05% in culture condition was A₃B₁C₃D₃E₂F₂G₁. The sequence of effect factor for hydrolysis of protein increase were temperature > culture time > cornmeal ration > inoculant concentration > wheat bran ration > wheat short ration > moisture. The highest hydrolysis of protein increase was 46.46% in culture condition was A₂B₃C₁D₃E₃F₃G₁. The increase rate of crude protein and hydrolysis of protein counted was positive relevance (R²=0.556).

Key words: Bacillus sublitis; solid fermentation; soybean meal; condition optimized