›› 2012, Vol. 39 ›› Issue (3): 60-64.

• 生物技术 • Previous Articles     Next Articles

Isolation, Identification and Genomic Sequencing of FS Mutation Strain of Porcine Reproductive and Respiratory Syndrome Virus

LI Jia-bin1,2, ZHAO Meng-meng1,2, LI Yu-gu2, MA Chun-quan1   

  1. 1. College of Life Science,Foshan University,Foshan 528231,China;2. College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China
  • Received:1900-01-01 Revised:2011-10-17 Online:2012-03-20 Published:2012-03-20

Abstract: A porcine reproductive and respiratory syndrome virus (PRRSV) strain isolated from visceral organs was designated as PRRSV-FS. The sequence analysis revealed that the isolated virus was a mutation strain as the Nsp2 gene existed amino acid deletion of 30 and the GP5 gene was conservative relatively. The genome of PRRSV FS strain showed 88.7%, 98.8%,58.5% of nucleotide indentity with ATCC VR-22332, JN-HS,Europena Lelystad virus respectively.

Key words: PRRSV; isolation and identification; genome; sequencing and analysis

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