Abstract: The embryo and ear marginal fibroblasts in Zaosheng cattle were isolated and identified in vitro using enzyme digestion and explant culture. The survival rate of ear marginal fibroblasts cultured with smaller explant were higher than that with bigger tissue. The growth curves of the embryo and ear marginal fibroblasts cultured with enzyme digestion were sigmoid type. The doubling time of embryo fibroblasts (29.7 h) was shorter than ear marginal bibroblast (31.2 h). The survival rate of defrozen embryo fibroblasts (96.8%) was higher than that of ear marginal fibroblasts (94.7%). The immunohistochemical staining was used to identify that intermediate filament proteins of isolated fibroblasts were stained tan, showing vimentin reaction is positive.

Key words: fibroblasts; explant culture; enzyme digestion; immunohistochemical staining