日本乙型脑炎病毒小鼠脑内分布研究

›› 2012, Vol. 39 ›› Issue (2): 225-227.

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Japanese Encephalitis Virus Distribution in the Brain of Mice

MA Li-min^1,2, LI Lin^1,2, ZHANG Ning^1,2, HU Le-peng^2,3, LI Ye¹, HE Ye¹, REN Xiu-mei¹, LI Zhi-ping^1,2, XUE Hui-liang², LI Dan², JIN Zhao¹, XIA Zhi-ping^2,3

1. College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China;2. Military Veterinary Institute,Academy of Military Medical Sciences of PLA,Changchun 130122,China;3. Jilin Agricultural Univesity,Changchun 130017,China

Received:1900-01-01 Revised:2011-12-16 Online:2012-02-20 Published:2012-02-20

Abstract

Abstract: To localize and detect the distribution of Japanese encephalitis virus (JEV) in the brain of experimental mouse,we established JEV infection model in mouse and produced paraffin sections using brain tissue. We established indirect immunohistochemical method with monoclonal antibodies of JEV to localize and detect the antigen. Experiment results showed that the method had a high sensitivity and specificity,it demonstrated that JEV infect neurons selectively,the antigen expression were mainly in the brainstem,ganglia and cortex.

Key words: Japanese encephalitis virus; indirect immunohistochemistry; antigen distribution

CLC Number:

S852.16

MA Li-min;LI Lin;ZHANG Ning;HU Le-peng;LI Ye;HE Ye;REN Xiu-mei;LI Zhi-ping;XUE Hui-liang;LI Dan;JIN Zhao;XIA Zhi-ping;. Japanese Encephalitis Virus Distribution in the Brain of Mice[J]. , 2012, 39(2): 225-227.

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Japanese Encephalitis Virus Distribution in the Brain of Mice

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