Abstract: To screen Chinese materia medica for prevention and cure embryo implantation dysfunction, mouse model with embryo implantation dysfunction was established by injected subcutaneously with mifepristone. Complex prescription composed of such Chinese materia medica as Salvia miltiorrhiza, Radix astragali, Szechwan lovage rhizome, Radix scutellariae, Rhizoma atractylodis macrocephalae, Semen cuscutae, Herba taxilli, Radix dipsaci, Radix codonopsis, Radix angelicae sinensis and some of them were evaluated in the model, pregnancy rate (PR), number of embryo implantation, progestin (P₄), estradiol (E₂) and ratio of progestin to estradiol (P₄/E₂) were the main index.The results of the screen test showed that PR was significantly higher in prescription 3 group than that in prescription 1 group, Semen cuscutae group and Radix dipsaci group (P＜0.05), and was higher than those in the prescription 2 group and control group, which indicated that prescription 3 was benefit to embryo implantation in mice. Pharmacodynamic test showed that PR was extreme significantly higher in tocolysis group than those in the control group and high-dose group (P＜0.01), and was significantly higher than that in the low-dose group (P＜0.05). Serum P₄ level and P₄/E₂ were extreme significantly higher in the tocolysis group than those in the high-dose group, low-dose group, control group and health group (P＜0.01), and P₄/E₂ was significantly higher in the tocolysis group than that in middle-dose group (P＜0.05), while E₂ level in serum was significantly lower in the tocolysis group, low-dose group and health group than that in the high-dose group and the control group (P＜0.05). P₄ level in serum was significantly higher in the middle-dose group than that in the control group (P＜0.05). In conclusion, prescription 3 could improve progestin level in serum and the ratio of progestin to estradiol in mice, which were helpful for embryo implantation.

Key words: embryo implantation dysfunction; Chinese materia medica; progestin; estradiol