绿壳蛋鸡IGF-Ⅰ基因SNPs分析及其与末期产蛋性能的相关性研究

Abstract

Abstract: Considering the association of IGF-Ⅰ with chicken reproductivity, IGF-Ⅰ gene was selected as a candidate to screen the nucleotide variation by PCR-RFLP method and analyze the association of polymorphisms with late egg reproduction. The genetic variation of the 5'region of IGF-Ⅰ gene was detected in 113 Green egg chickens by PCR-RFLP, and three genotypes(AA, BB and AB) were identified through PCR products cutting by PstⅠ restriction enzyme. By Chi square test, black feather Green egg chickens were in Hardy-Weinberg equilibrium at Pst Ⅰ site(P＞0.05). The statistic results showed that laying performance in the telophase of laying (14 and 15 months) was no significant difference between different genotypes(P＞0.05).

Key words: Green egg chickens; IGF-Ⅰ gene; laying performance; telophase of laying

CLC Number:

S814.8

CUI Zi-yang, YANG Hong-yan, ZHANG Yi-qiang, BAI Xiu-juan. Single Nucleotide Polymorpisms of IGF-Ⅰ Gene and Association with Late Egg Production in Green Egg Chickens[J]. , 2012, 39(12): 125-128.

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Single Nucleotide Polymorpisms of IGF-Ⅰ Gene and Association with Late Egg Production in Green Egg Chickens

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