猪11β-HSD1真核过表达载体的构建及其在Hepa1-6细胞中的表达

Abstract

Abstract: Glucocorticoids can be produced from inactive 11-keto forms through the enzyme 11β-hydroxysteroid dehydrogenase type 1. The cDNA of porcine 11β-HSD1 gene was amplified from the tissue of landrace pigs liver by RT-PCR and cloned into the eukaryotic expression vector pcDNA3.1. The recombinant was further identified by PCR amplification,enzyme digestion and DNA sequencing. The recombinant plasmid 11β-HSD1-pcDNA was transfected into the Hepa1-6 cell and its expression intensitye was detectable by RT-PCR. The results showed that the eukaryotic exprssion vector 11β-HSD1-pcDNA was successfully constructed which could provide a necessary basis for the research of transgenic pigs.

Key words: 11β-HSD1; eukaryotic expression; gene cloning; vector construction; transgenic

CLC Number:

XIA Ji-han, WANG Chao-qun, RUAN Jin-xue, YANG Shu-lin, LI Kui. Construction of 11β-HSD1 Eukaryotic Overexpression Vector and its Expression in Hepa1-6 Cell[J]. China Animal Husbandry & Veterinary Medicine, 2012, 39(12): 1-5.

References

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Construction of 11β-HSD1 Eukaryotic Overexpression Vector and its Expression in Hepa1-6 Cell

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