禽呼肠孤病毒S1733株单克隆抗体的制备及特性鉴定

Abstract

Abstract: The reovirus S1733 harvested from chicken embryos allantoic fluid was purified by sucrose gradient purification. The concentration was tested by protein testing kit. Then BALB/c mice were regularly immunized with purified reovirus S1733 contain 100 μg protein. After injecting four times, the spleen cells were collected and infused with myeloma cell SP2/0 follow ratio 5∶1. After selection in time and 3 times clone by limiting dilution. The two monoclonal antibodies were detected by indirect ELISA, Dot-ELISA, and immunofluorescence methods. The results showed that the purification protein was 43 mg/mL. Two hybridoma cells lines against reovirus named SF6-3K3 and SB2-1K3 were obtained. The titers were all above 10⁵ by indirect ELISA detection. It was not cross with others virus strains in this test by Dot-ELISA. The subtype was IgG1. It indicated that two hybridoma cell lines possessed the favorable specificity. Both of them could be used to detect reovirus in the future.

Key words: reovirus; monoclonal antibody; preparation; identification

CLC Number:

S852.65^＋9.4

XIE Zhi-qin, XIE Zhi-xun, LIU Jia-bo, PANG Yao-shan, DENG Xian-wen, XIE Li-ji, PENG Yi, FAN Qing. Preparation and Identification of Monoclonal Antibody against Reovirus S1733[J]. , 2012, 39(11): 47-51.

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Preparation and Identification of Monoclonal Antibody against Reovirus S1733

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