一例猪伪狂犬病病毒的分离鉴定

Abstract

Abstract: The brain tissue of piglet was obtained and the 217 bp long fragment in gp50 gene was amplified by the polymerase chain reaction (PCR). Therefore, the piglet was confirmed as porcine pseudorabies virus infection. Subsequently, the porcine pseudorabies virus was isolated on BHK-21 cells after 5 generations. The cells displayed typical cell lesions and were identified as porcine pseudorabies virus by PCR. The virus infectivety of the pseudorabies virus was 10^8.68 TCID₅₀/0.1 mL by Reed-Muench. Finally, the rabbits were inoculated with 10^7.0 TCID₅₀/mL virus. On 48 h the injection sites showed typical itching, skin damage and other symptoms. The rabbits were dead after 72 h by the inoculation of pseudorabies virus. Consequently, the results indicated that the isolated pseudorabies virus was highly pathogenicity to susceptible animals. It would be the research foundation of the virus epidemiology, pathogenesis,vaccine and diagnositic.

Key words: porcine pseudorabies virus; isolation and identification; PCR

CLC Number:

S852.65

BAO Yu-lun. The Isolation and Identification of the Pseudorabies Virus in pig[J]. , 2012, 39(11): 198-200.

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The Isolation and Identification of the Pseudorabies Virus in pig

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