羊膜对山羊角膜缘上皮细胞培养影响的研究

Abstract

Abstract: The differrnces of goat corneal limbal epithelial cells cultured respectively on intact amniotic membrane, denuded amniotic membrane and cryopreserved denuded amniotic membrane treated in different freezing time were assessed to find a optimum treatment method of amniotic membrane in this study. Limbal explant were cultured on the intact amniotic membrane, denuded amniotic membrane, uncryopreserved denuded amniotic membrane, 30-day cryopreserved denuded amniotic membrane, 90-day cryopreserved denuded amniotic membrane and 180-day cryopreserved denuded amniotic membrane respectively. The contrasts were performed among the growth characteristics and morphologies of corneal limbal epithelial cells seeded on different treated amniotic membrane. The results showed that the adherence and proliferation of cells cultured on denuded amniotic membrane were better than that of cells cultured on intact amniotic membrane. The cells cultured on both of the amniotic membranes could grow and form multilayer cellular structure. However, the keratinization of superficial cells cultured on the intact amniotic membrane was more than that of superficial cells cultured on denuded amniotic membrane. The adherence and proliferation of cells cultured on 30-day cryopreserved denuded amniotic membrane were better than that of cells cultured on uncryopreserved denuded amniotic membrane, 90-day cryopreserved denuded amniotic membrane and 180-day cryopreserved denuded amniotic membrane. The cells on all different treated amniotic membrane could grow and form multilayer cellular structure. However, no significant differences were found among the cellular structure of the different treated amniotic membrane. The 30-day cryopreserved denuded amniotic membrane was the optimum choice which could be used for reconstructing tissue engineering artificial cornea.

Key words: amniotic membrane; corneal limbal epithelial cells; culture in vitro

CLC Number:

S813.1^＋1

ZHAO Qing-mei, WANG Jian-li, DOU Zhong-ying. Effects of Amniotic Membrane on Goat Corneal Limbal Epithelial Cells Cultured in Vitro[J]. , 2012, 39(11): 116-121.

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Effects of Amniotic Membrane on Goat Corneal Limbal Epithelial Cells Cultured in Vitro

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