Abstract: One virus strain was isolated from the samples of brain and internal organs of a death piglet from a hogpen in Beijing. ST cell line shrunk, aggregated and detached from the culture system in 24 h approximately. The isolate could be neutralized by PRV standard positive serum. Rabbits were injected with the isolate and the typical clinical syndrome could be observed, such as extra tickle. According to the gD gene sequences of PRV in GenBank, a pair of primers was designed, a specific fragment was amplified by PCR, the results of sequencing analysis showed that the amplified fragment was the gene sequence of the PRV. All of the evidences indicated that the isolated virus was pseudorabies virus, and was named Beijing strain in accordance with separate location.

Key words: swine; pseudorabies virus; isolation; identification