China Animal Husbandry & Veterinary Medicine ›› 2012, Vol. 39 ›› Issue (1): 37-40.

• 生物技术 • Previous Articles     Next Articles

Construction of Eukaryotic Expression Vector of Laoshan Dairy Goat Myostatin and its Expression in the Fibroblast Cells

TANG Da-yun1,2,3, CHEN Xiao-liang1,2, WU Jian-min3, ZHU Hua-bin1, DU Wei-hua1, WANG Dong1, ZHAO Xue-ming1, CHEN Han-zhong2, LIN Xiu-kun1   

  1. 1. Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193,China;2. College of Animal Science and Technology,Guangxi University,Nanning 530004,China;3. Guangxi Veterinary Research Institute,Nanning 530001,China
  • Received:2011-05-25 Revised:1900-01-01 Online:2012-01-20 Published:2012-01-20

Abstract: This study aimed to clone cDNA of Myostatin of Laoshan dairy goat,and to construct Myostatin eukaryotic expression vector,analyzing its expression in the fibroblast cells. RNA was extracted from skeletal muscle and then reverse transcripted into cDNA. Myostatin was amplified by nested PCR. Myostatin was cloned into pcDNA3.1 to synthesize an eukaryotic expression vector,pcDNA-MSTN and its expression was detected by RT-PCR after transfected into the fibroblast cells. The results showed that the sequence of the full length cDNA of Myostatin gene of Laoshan dairy goat was similar with other species of goats and GenBank accession number was GU377303.1. The expression of Myostatin was significantly increased as detected by RT-PCR in fibroblast cells transfected with the expression vector. The study provided a basis for further study of the biological function of Myostatin and transgenic goat.

Key words: Laoshan dairy goat; Myostatin; eukaryotic expression vector; fibroblast; nested PCR

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