›› 2012, Vol. 39 ›› Issue (1): 159-163.

• 遗传繁育 • Previous Articles     Next Articles

Survey of Method on Individual Identification and Test about Breeding Oxen

LAI Shou-gui1,2, LIU Chou-sheng2, HE Gao-ming1, QIU Xiao-tian2, LIU Gang2, HAN Xu2   

  1. 1. Shihezi University, Shihezi 832003,China;2. National Center for Preservation and Utilization of Genetic Resources of Domestic Animals, National Animal Husbandry Service, Beijing 100193,China
  • Received:2011-04-08 Revised:1900-01-01 Online:2012-01-20 Published:2012-01-20

Abstract: This paper studied ingredients and concentration of multiple-primer PCR by orthogonal design and removed interference primer by permutation and combination. Result demonstrated that we successed to set up the system of multiple-primer PCR in reaction system which included 2.5 μL 10×Buffer(100 mmol/L Tris-HCl,500 mmol/L KCl, pH=9.7),8 mmol/L DDT,2.6 mmol/L MgCl2,0.8 mmol/L dNTP, 5% glycerol, 0.25% dimethy sulfoxide, 0.5% formamide and Taq polymerase 10 U. It could provide biology respond for interfering 11 pair primers. So it improved the efficiency of individual identification and superintend the marker of animal husbandry through against fake semen.

Key words: multiple-primer PCR; satellites; individual identification

CLC Number: