几种检测猪弓形虫病ELISA诊断试剂盒的对比

›› 2011, Vol. 38 ›› Issue (6): 218-221.

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Comparison of Five ELISA Kits for Detection of Anti-Toxoplasma gondii Antibodies in Experimentally Infected Swine

HE Yong¹, ZHU Yan-ping², YUAN Zi-guo¹, YIN Chuang-cheng¹, ZHOU Dong-hui¹, LIN Rui-qing¹, ZHU Xing-quan³, WENG Ya-biao¹

1. College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China;2. Department of Life Science and Technology,Xinxiang University,Xinxiang 453003,China;3. State Key Laboratory of Veterinary Etiological Biology,Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science,Lanzhou 730046,China

Received:2010-11-25 Revised:1900-01-01 Online:2011-06-20 Published:2011-06-20

Abstract

Abstract: The aim of the present study was to evaluate and compare the sensitivities of five ELISA kits for detection of Toxoplasma gondii infection in experimentally infected pigs. The PCR-positive serum samples from experimentally infected pigs were examined by the five ELISA kits. The results of different ELISA kits were compared among them and with that of PCR amplification to evaluate and compare their sensitivities. One ELISA kit (ELISA-A) made in China showed 100% detection of T. gondii antibody in experimentally infected pigs, showing 100% sensitivity. Whereas, the other ELISA kits displayed different low sensitivities. These results indicated that the ELISA-A kit is the most sensitive for detection of T. gondii infection in pigs.

Key words: Toxoplasma gondii; swine toxoplasmosis; experimental infection; ELISA; comparison

CLC Number:

S858.28

HE Yong;ZHU Yan-ping;YUAN Zi-guo;YIN Chuang-cheng;ZHOU Dong-hui;LIN Rui-qing;ZHU Xing-quan;WENG Ya-biao. Comparison of Five ELISA Kits for Detection of Anti-Toxoplasma gondii Antibodies in Experimentally Infected Swine[J]. , 2011, 38(6): 218-221.

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Comparison of Five ELISA Kits for Detection of Anti-Toxoplasma gondii Antibodies in Experimentally Infected Swine

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