Abstract: Three vectors were constructed，which were carrying firefly luciferase，driven by α-actin promoter，myosin light chain (mylpf) promoter and muscle creatine kinase (M-type，ckm) promoter，respectively.The three promoters were all from luxi cattle.The combined pRL-TK and three plasmids were transfected respectively into luxi cattle myoblasts and fibroblasts. After 72 h of transfection，dual luciferase reporter assay system was used to measure the activities of three promoters. We observed that the α-actin promoter provided luciferase reporter gene activity higher than mylpf promoter and ckm promoter in myoblasts and the luciferase reporter gene activities that were provided by the three promoters were similar to pGL3-Basic in fibroblasts，which certified that the three promoters were skeletal muscle-specific.This results provided an experimental basis for gene expression in skeletal muscle and establish the basis for gene efficient and specific expression in skeletal muscle.

Key words: bovine skeletal muscle-specific; promoter; myoblast; fibroblasts