鸡小肠上皮细胞的分离培养与鉴定

China Animal Husbandry & Veterinary Medicine ›› 2011, Vol. 38 ›› Issue (2): 68-71.

• 生物技术 • Previous Articles Next Articles

Culture of Chicken Intestine Epithelial Cells in vitro and its Characterization

LI Yan1, PENG Chun-yan1, LIANG Rong-wang2, ZHAO Guo-qi1, JIN Xiao-jun1

(1.College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China；2.Animal Husbandry and Veterinary Office of Boluo in Guangdong, Boluo 516100, China)

Received:1900-01-01 Revised:1900-01-01 Online:2011-02-20 Published:2011-02-20
Contact: ZHAO Guo-qi

Abstract

Abstract: In this study we selected tissue culture method to isolation chicken intestinal epithelial cells (IEC), using curettage, phase contrast digest and adherence methods to purify cells, with 0.05% Trypsin to digest and passage, immunocytochemical method to identify intestinal epithelial cells. The results showed that hadro-activity intestinal epithelial cells could be dissociated by tissue culture method, and obtain purified intestine epithelium. Morphology and immunocytochemical method showed that intestinal epithelial cells surface antigen demonstrate masculine, and purified intestine epithelium could be passaged in vitro.

Key words: chicken embryo; intestine epithelial cells; in vitro culture; characterization

CLC Number:

S811.2

LI Yan;PENG Chun-yan;LIANG Rong-wang;ZHAO Guo-qi;JIN Xiao-jun . Culture of Chicken Intestine Epithelial Cells in vitro and its Characterization[J]. China Animal Husbandry & Veterinary Medicine, 2011, 38(2): 68-71.

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Culture of Chicken Intestine Epithelial Cells in vitro and its Characterization

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