›› 2011, Vol. 38 ›› Issue (12): 61-63.

• 生理生化 • Previous Articles     Next Articles

Experimental Study on Parameter Optimization of Extraction Process of Leaf Protein from Clover

XIONG Chun-mei1, GUO Ai-wei2, CHEN Fen-fen2, WANG San-mei2   

  1. 1. Southwest Forestry University,Kunming 650224,China;2. College of Life Science,Southwest Forestry University,Kunming 650224,China
  • Received:2011-05-01 Revised:1900-01-01 Online:2011-12-20 Published:2011-12-20

Abstract: In this paper, the author select several common plants as the experimental materials,which are Clover. Then through Orthogonal Test L9(34),we partly optimize the parameters using the leaf protein concentrates (LPC) extraction technology,which are ratio of material to water,salt concentration,pH of extracting solution and coagulating temperature. we will obtain the optimized technological parameters in the leaf protein concentrates extraction technology. The results indicated that while in the orthogonal experiment,the ratio of clove meal to water,pH for extraction solution and coagulation temperature were affected LPC extraction ratio respectively. The optimum combination factor for clove extracting LPC were that 1∶3 for ratio of clove meal to water,5% for salt concentration,pH 3.0 for extraction solution and 70 ℃ for coagulation temperature,this research studied the optimum extraction technology of LPC by orthogonal test,so as to provide the theory for the development and application of LPC.

Key words: clover; leaf protein; extraction processing parameter

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