China Animal Husbandry & Veterinary Medicine ›› 2011, Vol. 38 ›› Issue (11): 214-217.

• 经验交流 • Previous Articles     Next Articles

Reconstruct the Contaminated BHK-21 Cells by Escherichia coli with Enrofloxacin

GAO Lin, LIAO De-fang, YAO Jun, ZHANG Nian-zu, LI Hua-chun, LI Zhi-hua   

  1. Key Laboratory of Tropical & Subtropical Animal Virology of the Ministry of Agriculture, Yunnan Tropical and Subtropical Animal Virus Diseases Laboratory,Yunnan Animal Science and Veterinary Institute,Kunming 650224,China
  • Received:2011-03-23 Revised:1900-01-01 Online:2011-11-20 Published:2011-11-20

Abstract: The aim is to reconstruct the BHK-21 cells contaminoted by Escherichia coli using Enrofloxacin.This study tested the MIC of Escherichia coli and BHK-21 cells,found the safe concentration of Enrofloxacin was 10 mg/L, it didn't effect the growth of BHK-21 cells, and could inhibit the Escherichia coli. By physical and chemical method, the contaminated BHK-21 cells were treated with the MEM growing solution of the same concentration of Enrofloxacin circularly. The Escherichia coli were cleaned successfully after 3 cycles and 3 passages, the BHK-21 cells were recovered. The method offered a reference for saving the contaminated cells.

Key words: Enrofloxacin; Escherichia coli; BHK-21 cells; clean contamination

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