›› 2010, Vol. 37 ›› Issue (8): 84-86.

• 遗传繁育 • Previous Articles     Next Articles

he Effect of Different Factors on the Development of Embryos from in vitro Fertilization of Sheep

WANG Li-qin1，ZHAO Yun-cheng1，CHEN Tong1，CHEN Shi-bin2，ZUO Bei-yao3，PENG Xia-yu4，HUANG Jun-cheng1，GUO Zhi-qin1，   

  1. (1. Key Laboratory of Animal Biotechnology of Xinjiang，Key Laboratory of Grass Livestock Reproduction ＆Breed Biotechnology Ministry of Agriculture,Urumqi 830000, China;2.College of Animal and Veterinary,Xinjiang Agricultural University,Urumqi 830052,China;3. Center of Animal Embryo Engineering and Technology, Nanjing Agricultural University, Nanjing 210095,China;4.College of Animal Science and Technology， Shihezi University ,Shihezi 832000,China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-08-20 Published:2010-08-20
  • Contact: GUO Zhi-qin;HUANG Jun-cheng

Abstract: The study was conducted to determine the effect of diffent heat serum, diffent sheep individuals,sperm density,cumulus’effect and different culture media on the development of embryos from in vitro fertilization.The results showed that heat three days serum affect greatly the cleavage rate (CR) and the blastocyst rate (BR)（P＜0.01）;and the different sperm how ever they are freezed or fresh have significant affect on CR and BR. No significant differences in CR and BR were found both sperm densities of 2×106 to 6×106 sperms/mL.The cumulus of oocyte have no effect on IVF;there were no different effect on BR and CR of the culture media of SOF and CRLaa.

Key words: sheep; IVF; heat serum; cleavage rate; blastocyst rate

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