›› 2010, Vol. 37 ›› Issue (6): 78-83.

• 生物技术 • Previous Articles     Next Articles

Isolation of Swine Influenza A Viruses and Genetic Characterization for NA Genes of the Isolated Strains

HOU Shao-yi1, FENG Jian-yuan1, GAO Yong-rui2, LU Gui-juan2, LU You-fei2, XIAO Ai-huan1,
ZHANG Min-xiu1, LU Bing-xia1, HUANG Wei-jian1, ZHAO Wu3   

  1. (1.College of Animal Science and Technology, Guangxi University, Nanning 530005, China; 2.Guangxi Liyuan Biologic CoLtd，Nanning 530001,China; 3.Guangxi Veterinary Research Institute, Nanning 530001, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-06-20 Published:2010-06-20
  • Contact: HUANG Wei-jian

Abstract: Three swine influenza virus strains were isolated from 9-11-day SPF chicken eggs in our study of these, GXHZ isolation was subtyped as H1N2 by HI assay and RT-PCR, and GXLZ，GXXY as H3N2. We detected the EID50 of these isolated strains and performed inoculation to pig with GXHZ isolation. NA genes amplificated from 3 isolated strains showed that their nucleotide and amino acid sequences had highest homology to A/Sw/Hainan/1/2005 (H1N2) strain. Two substitution at 6 (K6→R6) and 20 (V20→M20) separately located on the amino acid cytoplasmic tail and nonpolar transmembrane domain of the NA molecules of isolated strains. Phylogenical analysis of the NA genes indicated that the NA genes of isolated strains were most closely related to H1N2 which derived from swine origin influenza virus. Moreover, three isolated strains were clustered with H3N2 strains isolated after 1996.

Key words: swine influenza virus; viral isolation; NA gene; analysis of genetic evolution

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