Abstract: According to the gene sequences in GenBank of APP apxⅣ A gene, one pairs of specific primer was designed for amplifying the specific fragments of apxⅣ A gene. After optimization of annealing temperature and primers concentrations, PCR was established for simultaneous detection of the APP. The specific bands of 600 bp was amplified. The sensitⅣity and specificity tests showed that the PCR was highly sensitⅣe in 50 CFU/mL. No band was amplified from Staphyloccocus aureus Rosenbach, Streptococcus, Pasteurella multocida, Salmonella typhimurium, Haemophilus parasuis, Escherichia coli by PCR, which could be stored over 12 months at -20 ℃. 41 clinical samples were detected by the PCR, which were coincided with their bacteriological test. The results revealed that the established PCR assay was sensitⅣe，specific and reproducible and it could be used to detect rapidly APP.

Key words: Actinobacillus pleuropneumoniae; PCR detection Kit; apxⅣ A gene