Abstract: N protein is the major forming element of nucleocapsid of CDV and is also a conservative immunogenicity protein. A digoxin labeled probe prepared by enzyme digesting recombinant plasmid of CDV N protein and gel reclaiming targeting DNA was used to detect blenna narium of 40 suspected cases of CD and to detect spleen, liver, heart, lung, kidney and blood of 20 dead cases of CD by dot blotting hybridization. Results showed that among 40 suspected cases of CD, 33 cases were positive and positive rate was 82.5%. This rate was higher than that of colloid gold test paper detection. Virus relevance ratio of spleen was the highest among different tissues of detection. Above results showed that dot blotting hybridization had very important application value in early diagnosis and epidemiological investigation of CDV.

Key words: dot blotting hybridization; CDV; N gene