鸡约氏乳杆菌的分离鉴定及进化分析

›› 2010, Vol. 37 ›› Issue (1): 74-76.

• 生物技术 • Previous Articles Next Articles

Isolation and Identification and Evolution Analysisof Lactobacillus johnsonii in Chicken

ZHANG Feng-hua¹, XIA Ping-an¹, CUI Bao-an¹, ZHANG Hong-ying¹,GUO Guo-fu²

(1.Henan Agricultural University, College of Animal Science and Veterinary Medicine, Zhengzhou 450002, China; 2. Henan Entry-Exit Inspection and Quarantine Bureau of PRC, Zhengzhou 450003, China)

Received:1900-01-01 Revised:1900-01-01 Online:2010-01-20 Published:2010-01-20
Contact: XIA Ping-an

Abstract

Abstract: A Lactobacillus was isolated from three days chicken’s intestinal tract. Then, the 16S rRNA gene was amplified and analyzed by PCR method, and the PCR product was cloned and sequenced. A sequence of 1340 base pair (bp) was obtained from the bacterium, and it was published on the GenBank, the location is EU290749. The sequence homology and evolution relationship between the isolated bacterium and other Lactobacillus were analyzed, its sequence homology was 99.6% with the Lactobacillus johnsonii (AB295648) that was published on the GenBank. The isolated bacterium was identified as chicken Lactobacillus johnsonii, it was named HN/0711. It may be the suitable strain of microeco praeparatum.

Key words: Lactobacillus; isolation and identification; 16S rRNA; evolution analysis

CLC Number:

S831.7

ZHANG Feng-hua;XIA Ping-an;CUI Bao-an;ZHANG Hong-ying;GUO Guo-fu. Isolation and Identification and Evolution Analysisof Lactobacillus johnsonii in Chicken[J]. , 2010, 37(1): 74-76.

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Isolation and Identification and Evolution Analysisof Lactobacillus johnsonii in Chicken

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