›› 2010, Vol. 37 ›› Issue (1): 28-31.

• 生理生化 • Previous Articles     Next Articles

Purification and Partial Characterisation of Feruloyl Esterase from Barley Malt

LIU Sha-sha1, LI Hua2, SHI Qing-hua1, WANG Xi-dong1, CHENG Fa-xiang3   

  1. (1.College of Agronomy Xinjiang Agricultural University, Urumqi 830052,China; 2.China Agricultural University（Yantai）,Yantai 264670, China; 3.Laboratory of Animal Nutrition, College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-01-20 Published:2010-01-20
  • Contact: LI Hua

Abstract: The activity of feruloyl esterase has been enhanced in barley grain and barley malt.Feruloyl esterase solution was extracted from barley malt has been purified by ammonium sulfate precipitation，DEAE-cellulose column chromatography.The feruloyl esterase was purified 34-fold as shown in SDS-PAGE gel electrophoresis and the molecular weight estimated to be about 29.3 kD.Characterization of the enzyme exhibited that the optimum pH and the optimum temperature were 5.5 and 50 ℃，respectively，and it lost little activity after one hour below 60 ℃ from pH 4.5 to 7.5.It was inhibited by Zn2+，Fe3+，Cu2+，and strongly activated by EDTA and Na+. 

Key words: barley malt; feruloyl esterase; isolation and purification; enzyme characterisation

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