Abstract: The objective is to establish a simple, convenient, accurate, specific method to detect the NDV and detect the location of NDV in the COS-7 by comparing the indirect HRP-immunocytochemistry and indirect fluorescence immunocytochemistry. Both two experiment methods can detect the location of NDV and it was in the cell plasm of the COS-7. The indirect HRP-immunocytochemistry request the dilution of the first antibody is less than 1∶100, but the indirect fluorescence immunocytochemistry request the dilution of the first antibody is less than 1∶100. The indirect HRP-immunocytochemistry request the dilution of the second antibody is less than 1∶300, but the indirect fluorescence immunocytochemistry request the dilution of the second antibody is less than 1∶50. Further more, when detect the results that the indirect HRP-immunocytochemistry is more convenient than the indirect fluorescence immunocytochemistry. The indirect HRP-immunocytochemistry is simple, convenient, accurate and specific method to detect the NDV. 

Key words: immunoenzymatic celluar chemistry; immunofluorescence celluar chemistry; indirect; NDV; antigen localization