Abstract:

To investigate the mechanism of action of anti-inflammatory and immunological regulation, the change of interleukin-2〖JP〗〖HT〗〖HJ〗〖LM〗

〖HT5”BZ〗
interleukin-2 gene in the level of mRNA in the spleen of healthy and immunosuppressive mouse was detected with the technology of real time RT-PCR. Compared to the control group, the gene expression of interleukin-2 in the spleen of mouse both high dose （0.20 g/kg body weight） and low does TCDCA（0.10 g/kg body weight）group could raised（P<0.01）. Immunosuppressive model groups, high and low TCDCA could markedly revive the gene expression of interleukin-2 inhibited by ciclosporin A and the low TCDCA group markedly toped the control group. The mechanism of anti-inflammatory and immunoregulation has an intimate relationship with enhancing the gene expression of interleukin-2 inhibited in the level of mRNA in the spleen of mouse.

Key words: taurochenodeoxycholic acid; real time RT-PCR; interleukin-2