›› 2009, Vol. 36 ›› Issue (3): 102-105.

• 遗传繁育 • Previous Articles     Next Articles

Effect of Different Durations on Nuclear Maturation in Vitro of Feline Oocytes

WU Jing1，2, NI He-min2, LIU Yun-hai2, GUO Yong2, BAO Wen-yu2, 
XU Yang2, WANG Xin-zhuang3，1   

  1. (1. College of Veterinary Medicine, Northwest A&F University, Yangling 712100,China;  2.Department of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206,China;  3.Henan University of Agriculture, Zhengzhou 450002, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-03-20 Published:2009-03-20
  • Contact: WANG Xin-zhuang

Abstract: The aim of this study is to investigate the effect of different durations on nuclear maturation in vitro of feline oocytes.Following the in vitro maturation (IVM) in Medium 199 added with FSH and LH, feline oocytes were treated for 24 h, 28 h, 32 h, 36 h and 43 h respectively. Then, the polar body 1(PB1) were observed and oocytes were stained by aceto-orcein solution, and those with Pb1 and stayed at MⅡ phase, were considered as nuclear maturation. The rate of nuclear maturation in the 43 h group was significantly higher than the others (P<0.01). The rate of nuclear maturation in the 24 h group was significantly lower than the others (P<0.01), but there was no significant differences of the rate of nuclear maturation among other three groups（P>0.05）. In addition, the rate of degeneration in the 43 h group was also significantly higher than the others (P<0.01).Therefore， the optimal nuclear maturation of feline oocytes appeared following the duration from 28 to 36 h by IVM.

Key words: domestic cat; oocyte; in vitro nuclear maturation; duration

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